Category: Research highlight

S1P-mediated G-protein and RhoGTPase signaling to control endothelial barrier function

The bioactive sphingolipid S1P is present in bloodplasma, bound to carrier proteins, and is involved in many physiological processes, including angiogenesis, inflammatory responses and vascular stabilization. S1P can bind to several G-protein-coupled receptors (GPCRs) activating a number of different signalling

Research highlight: Bright red fluorescent protein created

After years of trying, biologists have succeeded in creating an extremely bright red fluorescent protein in the lab. This is good news for researchers, including cancer and stem cell researchers, who use fluorescent proteins to track essential cellular processes. The

Research highlight: Subcellular location of signaling proteins determines cell shape

The small GTPase RhoA is best known for its ability to remodel the actin cytoskeleton of cells.  To study the mechanism underlying the spatiotemporal control of RhoA activity by GEFs, single cell imaging was performed with an improved FRET sensor reporting on the nucleotide loading

Research highlight: Taming the image background beast

The quality of super resolution images obtained by stochastic single-molecule microscopy critically depends on image analysis algorithms. We find that the choice of background estimator is often the most important determinant of reconstruction quality. A variety of techniques have found

Research highlight: Annexin A4 dynamics

Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport; however, its precise